MON 95275 was produced by Agrobacterium-mediated transformation of maize tissue using the transformation vector PV-ZMIR525664. This vector contains a single T-DNA (transfer DNA), that is delineated by Right and Left Border regions. The T-DNA, contains the DvSnf7.1 suppression cassette, and the mpp75Aa1.1 and vpd4Da2 expression cassettes.